Nitrification in the Schelde estuary: methodological aspects and factors influencing its activity
de Bie, M.J.M.; Starink, M.; Boschker, H.T.S.; Peene, J.J.; Laanbroek, H.J. (2002). Nitrification in the Schelde estuary: methodological aspects and factors influencing its activity. FEMS Microbiol. Ecol. 42(1): 99-107. https://dx.doi.org/10.1111/j.1574-6941.2002.tb00999.x
We present a 15-month dataset on nitrification measurements in the Schelde estuary (Belgium and The Netherlands). Nitrification was estimated using the N-serve sensitive dark [14]C-bicarbonate incorporation technique. A peak of nitrification activity was observed in the freshwater part of the estuary. Downstream from this peak, nitrification declined, probably because of ammonium limitation. A range of nitrification inhibitors was tested on both a Nitrosomonas europaea culture and estuarine samples. It was found that methyl fluoride and acetylene stimulated dark [14]C-bicarbonate incorporation and those inhibitors were therefore considered inappropriate nitrification inhibitors in combination with this technique. The effect of the inhibitor N-serve was studied on the dark incorporation of [13]C-bicarbonate into polar lipid derived fatty acids to further identify the dominant chemoautotrophic processes. Inhibition of polar lipid derived fatty acid labelling in the presence of N-serve was complete, suggesting that nitrifying bacteria dominated the chemoautotrophic community.
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