A method for determining exoproteolytic activity in natural waters
Somville, M.; Billen, G. (1983). A method for determining exoproteolytic activity in natural waters. Limnol. Oceanogr. 28(1): 190-193. http://dx.doi.org/10.4319/lo.1983.28.1.0190
A very sensitive method allowing determination of exoproteolytic activity in natural water is based on the use of aminoacyl-ß-naphthylamide, which gives rise to a fluorescent product upon hydrolysis of its peptidelike bond by filtered or unfiltered water samples. Specificity of the method to exoproteases is demonstrated by a strong competitive inhibition effect of added proteins.A good correlation is found between exoproteolytic activity and the rate of amino acid utilization by microheterotrophs in various aquatic environments.
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy