Cellular energy allocation and scope for growth in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following chlorpyrifos exposure: a method comparison
Verslycke, T.; Roast, S.D.; Widdows, J.; Jones, M.B.; Janssen, C.R. (2004). Cellular energy allocation and scope for growth in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following chlorpyrifos exposure: a method comparison. J. Exp. Mar. Biol. Ecol. 306(1): 1-16. dx.doi.org/10.1016/j.jembe.2003.12.022
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| Keywords |
Aquatic organisms
Biology > Physiology
Biology > Physiology > Animal physiology
Energy
Environments > Aquatic environment > Brackishwater environment
Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine crustaceans
Food absorption
Indicators > Pollution indicators
Natural populations
Oxygen consumption
Pesticides
Population functions > Growth
Properties > Biological properties > Toxicity
Reproduction
Respiration
Tests > Toxicity tests
Toxicants
Toxicity testing
Crustacea [WoRMS]; Mysida [WoRMS]; Neomysis integer (Leach, 1814) [WoRMS]; Neomysis integer (Leach, 1814) [WoRMS]
Brackish water |
| Author keywords |
Neomysis integer; scope for growth; cellular energy allocation; biomarker; energy metabolism |
| Authors | | Top |
- Verslycke, T., more
- Roast, S.D.
- Widdows, J., more
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- Jones, M.B.
- Janssen, C.R., more
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| Abstract |
Mysids (Crustacea: Mysidacea) are used routinely in acute toxicity testing to evaluate the comparative toxicity of chemicals to aquatic organisms. The need for sublethal endpoints that provide comprehensive understanding of the potential impacts of toxicants to natural populations has resulted in examination of several physiological responses in mysid shrimp, including scope for growth (SFG) and cellular energy allocation (CEA). Both assays, based on the concept that energy in excess of that required for normal maintenance will be available for growth and reproduction, have been reported independently for the mysid Neomysis integer. The present study compares the responses of N. Integer following exposure to environmentally realistic concentrations of the organophosphate pesticide chlorpyrifos using both assays. Oxygen consumption in the SFG assay was significantly correlated with cellular respiration rate in the CEA assay, and both were significantly increased by chlorpyrifos exposure. In addition, the protein, sugar, lipid and total energy content in the CEA assay and the egestion rate in the SFG assay were significantly different in chlorpyrifos-exposed mysids compared with control mysids. In contrast, absorption efficiency in the SFG assay was unaffected by pesticide exposure. Significant effects in the SFG and CEA assays were more pronounced following short (i.e. 48 h) compared with longer exposure periods (e.g. 168 h). SFG was significantly reduced at near-lethal concentrations (0.072 and 0.100 µg chlorpyrifos l-1), whereas CEA was reduced in all chlorpyrifos- exposed mysids (0.038, 0.056, 0.072 and 0.100 µg chlorpyrifos l-1) although there was no concentration response. |
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