Een nieuwe methode voor het traceren van stikstof- en koolstofstromen door bacteriën en algen in aquatische microbiële gemeenschappen: Analyse van 15N- en 13C-incorporatie in D-alanine en andere hydrolyseerbare aminozuren = A new method for tracing flows of nitorgen and carbon through bacteria and algae in aquatic microbial communities : Analysis of 15N- and 13C-incorporation into D-alanine and other hydrolysable amino acids
Veuger, B. (2006). Een nieuwe methode voor het traceren van stikstof- en koolstofstromen door bacteriën en algen in aquatische microbiële gemeenschappen: Analyse van 15N- en 13C-incorporatie in D-alanine en andere hydrolyseerbare aminozuren = A new method for tracing flows of nitorgen and carbon through bacteria and algae in aquatic microbial communities : Analysis of 15N- and 13C-incorporation into D-alanine and other hydrolysable amino acids. NIOO Thesis, 47. PhD Thesis. Universiteit Utrecht: Utrecht. ISBN 90-5744-129-2. 153 pp.
This PhD-thesis deals with the development and application of a new method for analysis of uptake of nitrogen and carbon by bacteria and algae in water and sediment (the sea bottom). The method concerns analysis of uptake of the stable isotopes 15N and 13C (representing “common” nitrogen and carbon as they occur in nature) in amino acids. Since amino acids are a major component of all organisms, the total uptake of 15N and 13C in amino acids provides a good indication of total uptake by all (micro)organisms together. In addition, one of the amino acids (D-alanine) is only present in bacteria which means that uptake of 15N and 13C into D-alanine can be used to calculate uptake by bacteria. The method proved to be successful and application in various studies (on locations ranging from the Dutch Scheldt Estuary to the Australian east coast) yielded interesting information on the uptake and retention of nitrogen and carbon by bacteria and algae in sediments. Results from this research add to our understanding of the nitrogen cycle in water and sediment. This knowledge can for example be applied in water quality improvement work.
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